Follow-up report No. 12
See also: 9 December 2005, 2 December 2005, 2 December 2005, 25 November 2005, 18 November 2005, 4 November 2005, 4 November 2005, 28 October 2005, 21 October 2005, 21 October 2005, 14 October 2005, 14 October 2005
Information received on 12 and 15 December 2005 from Dr Gabriel Predoi, Director General, National Sanitary Veterinary and Food Safety Authority, Bucharest:
End of previous report period: 8 December 2005 (see Disease Information, 18 [49], 497, dated 9 December 2005).
End of this report period: 15 December 2005.
Identification of agent: highly pathogenic avian influenza (HPAI) virus serotype H5.
Date of first confirmation of the event: 7 October 2005.
Date of start of the event: 4 October 2005.
Nature of diagnosis: clinical and laboratory.
Confirmation of outbreak:
The suspected outbreak in the village of Crisan (Crisan district, Tulcea county), which was reported in Follow-up Report No. 11, was confirmed by the laboratory(1).
New outbreaks:
Description of affected population in the new outbreaks: backyard flocks.
Diagnosis:
Laboratory where diagnostic tests were performed Outbreak Samples examined Diagnostic tests used Date Results
Institute for Diagnostics and Animal Health (national reference laboratory) Tataru village Samples were taken from 2 hens, as follows:
- 10 cloacal swabs and organs (brain, lungs, trachea, bowels, heart, liver, kidneys).
a. RT-PCR(3) for detection of specific viral genome;
b. rapid test for HPAI diagnosis;
c. virus isolation in SPF(2) embryonated eggs.
13 Dec. 2005 a. positive;
b. positive;
c. positive.
Goidesti village Samples were taken from 1 hen and 1 turkey hen, as follows:
- 10 cloacal swabs and 6 tracheal swabs from 1 hen and 1 turkey hen;
- organs (brain, lungs, trachea, bowels, heart, liver, kidneys) from 1 hen.
a. rapid test for the detection of HPAI viral antigen;
b. RT-PCR(3) for detection of specific viral genome;
c. virus isolation in SPF(2) embryonated eggs.
12 Dec. 2005 a. positive;
b. positive;
c. positive for all samples.
Padina village Samples were taken from poultry (12 ducks+7 hens), as follows:
- 105 cloacal swabs and 42 tracheal swabs from 12 ducks and 7 hens;
- organs (brain, lungs, trachea, bowels, heart, liver, kidneys) from 3 hens;
- 6 blood samples from ducks.
virus isolation in SPF(2) embryonated eggs 12 Dec. 2005 positive for all samples examined
Tonea village Samples were taken from 5 hens, as follows:
- 25 cloacal and tracheal swabs;
- organs (brain, lungs, trachea, bowels, heart, liver, kidneys).
a. rapid test for the detection of HPAI viral antigen;
b. RT-PCR(3) for detection of specific viral genome;
c. virus isolation in SPF(2) embryonated eggs.
13 Dec. 2005 a. positive;
b. positive;
c. positive.
Origin of infection: contact with wild birds.
Control measures
A. Undertaken:
- stamping out (outbreaks in Padina and Goidesti);
- partial stamping out (outbreaks in Tataru and Tonea);
- quarantine;
- movement control inside the country;
- screening;
- zoning;
- disinfection of infected premises/establishments.
B. To be undertaken:
- control of wildlife reservoirs.
Vaccination prohibited: no.
Other details/comments:
Outbreak (village) Control measures Epidemiology
Tataru Control measures have been applied in Tataru in the past few days: partial stamping out has been applied to the infected premises and to neighbouring backyard premises, in accordance with the legislative provisions in force (Ministry for Agriculture, Forestry and Rural Development Order No. 311, Art. 10). A total of 2,485 poultry were culled and destroyed. Corpses were destroyed by burning and burying. Backyard premises in the locality have been disinfected.
Movement control has been applied to people, poultry, poultry products, feed, etc.
There is a lake in immediate vicinity of the village which justifies the presence of migratory birds in the affected area.
Goidesti The application of control measures began on 11 December 2005. The outbreak appeared in a hamlet with only five households.
Padina The application of control measures began on 12 December 2005. The entire flock of Padina consists of almost 47,000 poultry. Stamping out will be applied to all birds within the affected locality. Culling of poultry will be performed by gassing with CO2 in containers. Corpses will be destroyed by burning and burying. Backyard premises and roads in the locality will be disinfected.
Movement control will be applied to all transport of people, poultry, poultry products, feed, etc.
In the affected area there are no lakes or pools that could account for the presence of migratory birds. The infection probably took place when a flock of migrating birds stopped to rest.
Tonea The application of control measures began on 14 December 2005. Partial stamping out is being applied in the infected backyard premises and in the immediate vicinity. A total of 375 poultry have been culled, and 29 premises have been disinfected.
Movement control has been applied to the entire village.
The outbreak appeared in a small village in the vicinity of Danube meadow land and some small lakes, alongside the corridors used by migrating birds. The main concern in this case is the presence of some large poultry farms around Modelu district.
Final report: no.
(1) Note by the OIE Animal Health Information Department: this information, which was received at the OIE Headquarters on 6 December 2005, was inadvertently omitted from Disease Information, 18 (49), 497-499.
(2) SPF: specific pathogen-free
(3) RT-PCR: reverse transcriptase - polymerase chain reaction
